Clodronate Liposome is used in different research laboratories. Clodronate is used as a treatment for different diseases, especially for cancer metastases. It effects further development of metastases, reduces pain and decreases the risk of fractures. Since this drug cannot be delivered to tissues without suppressing macrophage activity, this nano technology is used for this purpose.
To be closed in vivo, this effective compound is delivered encapsulated within liposomes. A liposome is lipid-based tiny particle, designed to carry different compounds in form of suspension. It is lately used in many research laboratories, as highly effective way or delivering drugs to various tissues. In this case, to bones and kidneys.
Manipulating clodronate liposome is associated with some risks, because it is highly irritating to skin, eyes, mucous membranes and respiratory system. It is very important to use appropriate clothing, chemical-resistant goggles and gloves and respirators. This suspension might be dangerous if swallowed or inhaled, and it shouldn't get in contact with your skin or eyes. It can be easily absorbed through skin.
In case of oral exposure, you should wash out mouth with water and call a physician. If your eyes were in contact with this compound, you should flush them with water for at least fifteen minutes. Dermal exposure should be treated with soap and water. If inhaled, it may cause breathing problems. Exposed person should get to fresh air, and sometimes it might be necessary to use respirator or even oxygen.
Suppression of macrophage function is necessary for delivering drug to tissues. Macrophages are an essential part of natural immune system, but they also participate in or control other functions. They ingest and digest different microorganisms, and that's why you need to encapsulate some compounds within liposomes, to get through this macrophage layer.
When clodronate is encapsulated within liposomes, it can be delivered to bones and kidneys. Macrophages will digest those particles, but this compound will ve released and accumulated in tissues. Creating macrophage depleted tissues and organs this way allows more successful research of macrophage functional aspects.
Different diseases can be researched this way, using clodronate liposome. Some autoimmune diseases, transplantation and neurological disorders can also be researched using this type of macrophage suppression. The same method can actually be used for in vitro depletion of macrophages, but it is primarily designed for in vivo researches.
It is important to keep the suspension at appropriate temperature. It shouldn't be exposed to temperatures above 30 degrees of Celsius, or frozen. The ideal storage temperature is four degrees Celsius, and in this case it can be kept one month in refrigerator. For achieving desired results, the suspension should be kept outside the refrigerator until it reaches room temperature, before injecting it to animals.
Always shake the suspension, other ways it won't be homogeneous, and you won't get desired results. If you use the same syringe for different animals, you won't get accurate dosages, and you might infect them. Always shave and cleanse the skin with alcohol before injecting clodronate liposome to your animal.
To be closed in vivo, this effective compound is delivered encapsulated within liposomes. A liposome is lipid-based tiny particle, designed to carry different compounds in form of suspension. It is lately used in many research laboratories, as highly effective way or delivering drugs to various tissues. In this case, to bones and kidneys.
Manipulating clodronate liposome is associated with some risks, because it is highly irritating to skin, eyes, mucous membranes and respiratory system. It is very important to use appropriate clothing, chemical-resistant goggles and gloves and respirators. This suspension might be dangerous if swallowed or inhaled, and it shouldn't get in contact with your skin or eyes. It can be easily absorbed through skin.
In case of oral exposure, you should wash out mouth with water and call a physician. If your eyes were in contact with this compound, you should flush them with water for at least fifteen minutes. Dermal exposure should be treated with soap and water. If inhaled, it may cause breathing problems. Exposed person should get to fresh air, and sometimes it might be necessary to use respirator or even oxygen.
Suppression of macrophage function is necessary for delivering drug to tissues. Macrophages are an essential part of natural immune system, but they also participate in or control other functions. They ingest and digest different microorganisms, and that's why you need to encapsulate some compounds within liposomes, to get through this macrophage layer.
When clodronate is encapsulated within liposomes, it can be delivered to bones and kidneys. Macrophages will digest those particles, but this compound will ve released and accumulated in tissues. Creating macrophage depleted tissues and organs this way allows more successful research of macrophage functional aspects.
Different diseases can be researched this way, using clodronate liposome. Some autoimmune diseases, transplantation and neurological disorders can also be researched using this type of macrophage suppression. The same method can actually be used for in vitro depletion of macrophages, but it is primarily designed for in vivo researches.
It is important to keep the suspension at appropriate temperature. It shouldn't be exposed to temperatures above 30 degrees of Celsius, or frozen. The ideal storage temperature is four degrees Celsius, and in this case it can be kept one month in refrigerator. For achieving desired results, the suspension should be kept outside the refrigerator until it reaches room temperature, before injecting it to animals.
Always shake the suspension, other ways it won't be homogeneous, and you won't get desired results. If you use the same syringe for different animals, you won't get accurate dosages, and you might infect them. Always shave and cleanse the skin with alcohol before injecting clodronate liposome to your animal.
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